MicroRNA-132 promotes atherosclerosis by inducing mitochondrial oxidative stressmediated ferroptosis / 南方医科大学学报

OBJECTIVE@#To explore the expression of microRNA-132 (miR-132) and its potential role in the development of atherosclerosis (AS).@*METHODS@#Thirty AS samples and 30 samples of normal peripheral vessels were collected from atherosclerotic patients undergoing peripheral angiostomy in our hospital for detecting the expression level of miR-132 using RT-qPCR. The expression of miR-132 in human umbilical vein endothelial cells (HUVEC) was up-regulated by liposome transfection, and intracellular reactive oxygen species (ROS), localization relationship between ROS and mitochondria, functional changes of mitochondrial reactive oxygen superoxide species (mtROS), mitochondrial membrane potential (MMP) and opening of mitochondrial permeability transition pore (mPTP) were analyzed by flow cytometry and laser confocal microscopy. The activity of mitochondrial redox respiratory chain complex (type I, II, III, IV and V) in HUVECs was detected using ELISA, and the expression levels of key iron death proteins were detected with Western blotting.@*RESULTS@#RT-qPCR results showed that miR-132 was significantly up-regulated in atherosclerotic plaques compared with normal vascular samples (P < 0.001). Compared with control HUVECs, HUVECs overexpressing miR-132 showed a significantly increased level of intracellular ROS (P < 0.001), and most of ROS was colocalized with mitochondria. HUVECs overexpressing miR-132 also showed significantly decreased MMP (P < 0.001) and obviously increased mtROS (P < 0.001) and opening of mPTP (P < 0.001), which led to mitochondrial REDOX respiratory chain stress disorder. The key iron death protein GPX4 was significantly down-regulated and the oxidized protein NOX4 was significantly increased in miR-132-overexpressing HUVECs (P < 0.001).@*CONCLUSION@#MiR-132 promotes atherosclerosis by inducing mitochondrial oxidative stress-mediated ferroptosis, which may serve as a promising therapeutic target for AS.

Occupational health monitoring status of medical radiation workers in Qinzhou City, Guangxi / 上海预防医学

Objective To understand the situation of occupational health monitoring status of medical radiation workers in radiation diagnosis and treatment institutions, to provide reference for the evaluation of radiation protection measures as well as for the health supervision/inspection agencies, and to maximize the protection of occupational health rights of medical workers in radiation diagnosis and treatment. Methods Questionnaires were distributed then collected in the radiation diagnosis and treatment institutions in Qinzhou. The data were analyzed afterwards. Results A total of 850 medical radiation workers were recruited from 101 institutions that had obtained the radiation diagnosis and treatment license in Qinzhou. Among them, the physical examination rate of radiation diagnosis and treatment staff was 96.94%, the personal dosimeter monitoring rate was 93.41%, the training rate of radiation protection and legal knowledge was 94.12%, and the monitoring rate of equipment performance stability was 51.49%. Conclusion The occupational health monitoring of radiation workers in high-level institutions relatively meets the standard, and the management of occupational health monitoring in township hospitals, private hospitals and dental clinics is not satisfactory. The monitoring rate of radiation equipment performance in the whole city is generally low. Health supervision and law enforcement departments should strengthen law enforcement, urge the institutions to conduct radiation occupational health monitoring in an orderly manner according to law, and protect the health rights/interests of radiation diagnosis and treatment workers to the maximum extent.

Carotid artery stenosis treated with modified carotid endarterectomy: report of two cases / 南方医科大学学报

Based on standard carotid endarterectomy, we performed modified carotid endarterectomy in two cases of carotid artery stenosis by changing the direction of the carotid artery incision to avoid restenosis of the internal carotid artery without using a patch. The two patients recovered smoothly without any complications. Compared with eversion or patch endarterectomy, this modified carotid endarterectomy avoids restenosis of the carotid artery and shortens operation time.